Publication Spotlights


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Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies

Jones HG, Ritschel T, Pascual G, Brakenhoff JPJ, Keogh E, Furmanova-Hollenstein P, Lanckacker E, Wadia JS, Gilman MSA, Williamson RA, Roymans D, van 't Wout AB, Langedijk JP, McLellan JS. PLoS Pathog. 2018 Mar 6;14(3):e1006935. PMID: 29509814
 

The attachment glycoprotein (RSV G) purportedly binds to CX3CR1 on human airway epithelial cells to mediate viral attachment and subsequent infection. This manuscript describes the characterization of two human antibodies that bind to the central conserved region of RSV G, potently neutralize both subtypes of RSV, and protect RSV-challenged animals from severe disease. We also determined high-resolution crystal structures of each antibody in complex with the central conserved region of RSV G to gain a better understanding of how these antibodies bind to RSV G and how they neutralize the virus. Comparison of these structures with the structure of fractalkine (CX3CL1 – the natural ligand of CX3CR1) suggests that RSV G likely binds to CX3CR1 in a mode that is distinct from that of fractalkine. Collectively, these results build on recent studies demonstrating the importance of RSV G in antibody-mediated protection, and the structural information should guide the development of new vaccines as well as the investigation of the structural interactions between RSV G and CX3CR1 that mediate viral attachment.


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Infants infected with respiratory syncytial virus generate potent neutralizing antibodies that lack somatic hypermutation

Goodwin E, Gilman MSA, Wrapp D, Chen M, Ngwuta JO, Moin SM, Bai P, Sivasubramanian A, Connor RI, Wright PF, Graham BS, McLellan JS, Walker LM. Immunity 2018; 48(2):339-349.e5.
PMID: 29396163




Respiratory syncytial virus (RSV) is a leading cause of infant mortality, and there are currently no licensed vaccines to protect this vulnerable population. This manuscript describes the isolation and characterization of more than 450 RSV fusion glycoprotein (F)-specific antibodies from 7 RSV-infected infants. These studies revealed that infant antibody repertoires are highly focused compared to those of adults, and that antibodies directed against two dominant sites utilize convergent sequence features for recognition of RSV F. A subset of antibodies targeting one of these sites displayed potent neutralizing activity despite lacking somatic mutations, suggesting that expansion of these B cells in infants may be possible with suitably designed vaccine antigens. Structural studies revealed the molecular determinants for the convergent recognition of both immunodominant sites, providing a framework for the rational design of age-specific RSV vaccines.


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Structure and immunogenicity of prefusion-stabilized human metapneumovirus F glycoprotein

Battles MB, Más V, Olmedillas E, Cano O, Vázquez M, Rodríguez L, Melero JA, McLellan JS. Nat Commun. 2017 Nov 16;8(1):1528.
PMID: 29142300

Human metapneumovirus (hMPV) is a leading cause of severe lower respiratory tract infections in children yet an effective vaccine is not available. This manuscript describes three major advances in hMPV vaccine development: (1) Rational design of the first prefusion-stabilized hMPV F protein; (2) The first X-ray crystal structure of trimeric hMPV F in the prefusion conformation, determined to 2.6 Å resolution, and (3)  Serological and immunization studies that demonstrate the specificity of antibodies elicited by hMPV F is strikingly different than for hRSV F.


Selected Publications

 

Tian D, Battles MB, Moin SM, Chen M, Modjarrad K, Kumar A, Kanekiyo M, Graepel KW, Taher NM, Hotard AL, Moore ML, Zhao M, Zheng ZZ, Xia NS, McLellan JS, Graham BS.
Structural basis of respiratory syncytial virus subtype-dependent neutralization by an antibody targeting the fusion glycoprotein.
Nat Commun. 2017 Nov 30;8(1):1877. 
PMID: 29187732
 

Pallesen J, Wang N, Corbett KS, Wrapp D, Kirchdoerfer RN, Turner HL, Cottrell CA, Becker MM, Wang L, Shi W, Kong WP, Andres EL, Kettenbach AN, Denison MR, Chappell JD, Graham BS, Ward AB, McLellan JS.
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen
PNAS 2017 Aug 29;114(35):E7348-E7357. 
PMID: 28807998
 

Rossey I, Gilman MS, Kabeche SC, Sedeyn K, Wrapp D, Kanekiyo M, Chen M, Mas V, Spitaels J, Melero JA, Graham BS, Schepens B, McLellan JS, Saelens X.
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state.
Nat Commun. 2017 Feb 13;8:14158.
PMID: 28194013
 

Gilman MS, Castellanos CA, Chen M, Ngwuta JO, Goodwin E, Moin SM, Mas V, Melero JA, Wright PF, Graham BS, McLellan JS, Walker LM.
Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors.
Sci Immunol. 2016 Dec 16;1(6). pii: eaaj1879.
PMID: 28111638
 

Más V, Rodriguez L, Olmedillas E, Cano O, Palomo C, Terron MC, Luque D, Melero JA, McLellan JS.
Engineering, Structure and Immunogenicity of the Human Metapneumovirus F Protein in the Postfusion Conformation.
PLoS Pathog. 2016 Sep 9;12(9):e1005859.
PMID: 27611367
 

Misasi J, Gilman MS, Kanekiyo M, Gui M, Cagigi A, Mulangu S, Corti D, Ledgerwood JE, Lanzavecchia A, Cunningham J, Muyembe-Tamfun JJ, Baxa U, Graham BS, Xiang Y, Sullivan NJ, McLellan JS.
Structural and molecular basis for Ebola virus neutralization by protective human antibodies.
Science. 2016 Mar 18;351(6279):1343-6.
PMID: 26917592
 

Kirchdoerfer RN, Cottrell CA, Wang N, Pallesen J, Yassine HM, Turner HL, Corbett KS, Graham BS, McLellan JS, Ward AB.
Pre-fusion structure of a human coronavirus spike protein.
Nature. 2016 Mar 3;531(7592):118-21.
PMID: 26935699
 

Battles MB, Langedijk JP, Furmanova-Hollenstein P, Chaiwatpongsakorn S, Costello HM, Kwanten L, Vranckx L, Vink P, Jaensch S, Jonckers TH, Koul A, Arnoult E, Peeples ME, Roymans D, McLellan JS.
Molecular mechanism of respiratory syncytial virus fusion inhibitors.
Nat Chem Biol. 2016 Feb;12(2):87-93.
PMID: 26641933
 

Krarup A, Truan D, Furmanova-Hollenstein P, Bogaert L, Bouchier P, Bisschop IJ, Widjojoatmodjo MN, Zahn R, Schuitemaker H, McLellan JS, Langedijk JP.
A highly stable prefusion RSV F vaccine derived from structural analysis of the fusion mechanism.
Nat Commun. 2015 Sep 3;6:8143
PMID: 26333350
 

Gilman MS, Moin SM, Mas V, Chen M, Patel NK, Kramer K, Zhu Q, Kabeche SC, Kumar A, Palomo C, Beaumont T, Baxa U, Ulbrandt ND, Melero JA, Graham BS, McLellan JS.
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein.
PLoS Pathog. 2015 Jul 10; 11(7):e1005035.
PMID: 26161532

 

View all of Dr. McLellan's publications on PubMed